KMID : 1140220140190040279
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´ëÇѾϿ¹¹æÇÐȸÁö 2014 Volume.19 No. 4 p.279 ~ p.287
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Anti-inflammatory Effects of Schisandra chinensis (Turcz.) Baill Fruit Through the Inactivation of Nuclear Factor-¥êB and Mitogen-activated Protein Kinases Signaling Pathways in Lipopolysaccharide-stimulated Murine Macrophages
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Kang Young-Soon
Han Min-Ho Hong Su-Hyun Park Cheol Hwang Hye-Jin Kim Byung-Woo Kim Ho-Kyoung Choi Young-Whan Kim Cheol-Min Choi Yung-Hyun
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Abstract
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Background: Schisandrae Fructus, the dried fruit of Schisandra chinensis (Turcz.) Baill. (Magnoliaceae), is widely used in traditional medicine for the treatment of a number of chronic inflammatory diseases. This study examined the anti-inflammatory effects of Schisandrae Fructus ethanol extract (SF) on the production of pro-inflammatory substances in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages.
Methods: To measure the effects of SF on pro-inflammatory mediator and inflammatory cytokine¡¯s expression and production in RAW 264.7 cells, we used the following methods: cell viability assay, Griess reagent assay, enzyme-linked immunosorbent assay, reverse transcriptase-polymerase chain reaction, Western blotting analysis and immunofluorescence staining.
Results: Stimulation of the RAW 264.7 cells with LPS caused an elevated production of nitric oxide (NO), tumor necrosis factor ¥á (TNF-¥á) and interleukin (IL)-1¥â, which was markedly inhibited by the pretreatment with SF without causing any cytotoxic effects. SF also inhibited the expression of inducible NO synthase, TNF-¥á, and IL-1¥â protein and their mRNAs in LPS-stimulated RAW 264.7 cells. Furthermore, SF attenuated LPS-induced nuclear translocation of nuclear factor-¥êB (NF-¥êB) by reducing inhibitory-¥êB degradation, and reduced the phosphorylation of mitogen-activated protein kinases (MAPKs), implying that SF regulated LPS-induced NF-¥êB-dependent inflammatory pathways through suppression of MAPKs activation.
Conclusions: SF may be useful for the treatment of various inflammatory diseases.
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KEYWORD
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Schisandra chinensis, Anti-inflammation, Nuclear factor-kappa B, Mitogen-activated protein kinases
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